| Longnose Sucker | Catostomus | catostomus | | 6 | Catostomidae | Cypriniformes | Actinopterygii | Animalia |
| Redside Dace | Clinostomus | elongatus | | 2 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Bridle Shiner | Notropis | bifrenatus | | 6 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Cheat Minnow | Pararhinichthys | bowersi | | 2 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Maryland Darter | Etheostoma | sellare | | 8 | Percidae | Perciformes | Actinopterygii | Animalia |
| Trout-perch | Percopsis | omiscomaycus | | 5 | Percopsidae | Percopsiformes | Actinopterygii | Animalia |
| Mudpuppy | Necturus | maculosus | | 4 | Proteidae | Caudata | Amphibia | Animalia |
| Greater Prairie-Chicken | Tympanuchus | cupido | | 1 | Phasianidae | Galliformes | Aves | Animalia |
| Lark Sparrow | Chondestes | grammacus | | 86 | Emberizidae | Passeriformes | Aves | Animalia |
| Bachman's Sparrow | Peucaea | aestivalis | | 15 | Emberizidae | Passeriformes | Aves | Animalia |
| Bewick's Wren | Thryomanes | bewickii | | 29 | Troglodytidae | Passeriformes | Aves | Animalia |
| Olive-sided Flycatcher | Contopus | cooperi | | 115 | Tyrannidae | Passeriformes | Aves | Animalia |
| Red-cockaded Woodpecker | Dryobates | borealis | | 8 | Picidae | Piciformes | Aves | Animalia |
| Maryland Glyph | Glyphyalinia | raderi | | 1 | Zonitidae | Stylommatophora | Gastropoda | Animalia |
| No Common Name | Monoxia | obesula | | 1 | Chrysomelidae | Coleoptera | Insecta | Animalia |
| Schwarz's Diving Beetle | Laccophilus | schwarzi | | 1 | Dytiscidae | Coleoptera | Insecta | Animalia |
| American Burying Beetle | Nicrophorus | americanus | | 2 | Silphidae | Coleoptera | Insecta | Animalia |
| Golden-banded Skipper | Telegonus | cellus | | 5 | Hesperiidae | Lepidoptera | Insecta | Animalia |
| Hessel's Hairstreak | Callophrys | hesseli | | 3 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Dusky Azure | Celastrina | nigra | | 3 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| Regal Fritillary | Speyeria | idalia | | 34 | Nymphalidae | Lepidoptera | Insecta | Animalia |
| Skillet Clubtail | Gomphurus | ventricosus | | 4 | Gomphidae | Odonata | Insecta | Animalia |
| Piedmont Clubtail | Hylogomphus | parvidens | | 4 | Gomphidae | Odonata | Insecta | Animalia |
| Riverine Clubtail | Stylurus | amnicola | | 1 | Gomphidae | Odonata | Insecta | Animalia |
| No Common Name | Caecidotea | nordeni | | 3 | Asellidae | Isopoda | Malacostraca | Animalia |
| American Bison | Bison | bison | | 1 | Bovidae | Artiodactyla | Mammalia | Animalia |
| Elk | Cervus | elaphus | | 1 | Cervidae | Artiodactyla | Mammalia | Animalia |
| Gray Wolf | Canis | lupus | | 2 | Canidae | Carnivora | Mammalia | Animalia |
| Mountain Lion | Puma | concolor | | 0 | Felidae | Carnivora | Mammalia | Animalia |
| American Marten | Martes | americana | | 0 | Mustelidae | Carnivora | Mammalia | Animalia |
| Snowshoe Hare | Lepus | americanus | | 10 | Leporidae | Lagomorpha | Mammalia | Animalia |
| Black-tailed Jackrabbit | Lepus | californicus | | 3 | Leporidae | Lagomorpha | Mammalia | Animalia |
| Eastern Harvest Mouse | Reithrodontomys | humulis | | 5 | Muridae | Rodentia | Mammalia | Animalia |
| Rock Clubmoss | Huperzia | porophila | | 2 | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Great Angelica | Angelica | atropurpurea | | 5 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| Button Eryngo | Eryngium | yuccifolium | | 4 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| Canadian Licorice-root | Ligusticum | canadense | | 2 | Apiaceae | Apiales | Magnoliopsida | Plantae |
| Spanish Moss | Tillandsia | usneoides | | 1 | Bromeliaceae | Asparagales | Magnoliopsida | Plantae |
| Great Indian Plantain | Arnoglossum | reniforme | | 7 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Sea Oxeye | Borrichia | frutescens | | 3 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Spotted Joe Pye Weed | Eutrochium | maculatum | | 31 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Heller's Blazing Star | Liatris | helleri | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Bushy Knotweed | Polygonum | ramosissimum | | 16 | Polygonaceae | Caryophyllales | Magnoliopsida | Plantae |
| Golden Colicroot | Aletris | aurea | | 3 | Nartheciaceae | Dioscoreales | Magnoliopsida | Plantae |
| Beetleweed | Galax | urceolata | | 3 | Diapensiaceae | Ericales | Magnoliopsida | Plantae |
| Sidebells Wintergreen | Orthilia | secunda | | 15 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Green-flowered Wintergreen | Pyrola | chlorantha | | 13 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Eastern Trailing Tick-trefoil | Desmodium | humifusum | | 1 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Sand Tick-trefoil | Desmodium | lineatum | | 12 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Sessileleaf Tick-trefoil | Desmodium | sessilifolium | | 10 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Sampson's Snakeroot | Orbexilum | pedunculatum | | 0 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Buffalo Clover | Trifolium | reflexum | | 7 | Fabaceae | Fabales | Magnoliopsida | Plantae |
| Downy Gentian | Gentiana | puberulenta | | 2 | Gentianaceae | Gentianales | Magnoliopsida | Plantae |
| Slender Watermilfoil | Myriophyllum | tenellum | | 4 | Haloragaceae | Haloragales | Magnoliopsida | Plantae |
| Slender Waternymph | Najas | gracillima | | 7 | Hydrocharitaceae | Hydrocharitales | Magnoliopsida | Plantae |
| American Beautyberry | Callicarpa | americana | | 0 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Nettle-leaved Sage | Salvia | urticifolia | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Blunt-lobed Gerardia | Agalinis | obtusifolia | | 38 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| American Bluehearts | Buchnera | americana | | 22 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Chaffseed | Schwalbea | americana | | 2 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| Tropical Waterhyssop | Bacopa | innominata | | 4 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Branched Hedgehyssop | Gratiola | ramosa | | 1 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Heart-leaved Plaintain | Plantago | cordata | | 1 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Dwarf Plantain | Plantago | pusilla | | 2 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Wood Lily | Lilium | philadelphicum | | 10 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Coastal False Asphodel | Triantha | racemosa | | 7 | Tofieldiaceae | Liliales | Magnoliopsida | Plantae |
| Dragon's Mouth Orchid | Arethusa | bulbosa | | 6 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Showy Lady's Slipper | Cypripedium | reginae | | 2 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Lesser Rattlesnake Plantain | Goodyera | repens | | 2 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Checkered Rattlesnake Plantain | Goodyera | tesselata | | 3 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Small Whorled Pogonia | Isotria | medeoloides | | 6 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Heartleaf Twayblade | Neottia | cordata | | 2 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Variable Sedge | Carex | polymorpha | | 1 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Quill Sedge | Carex | tenera | | 3 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Toothed Flatsedge | Cyperus | dentatus | | 3 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Feather-bristled Beaksedge | Rhynchospora | oligantha | | 2 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Pale Beaksedge | Rhynchospora | pallida | | 1 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Smith's Bulrush | Schoenoplectiella | smithii | | 15 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Canby's Bulrush | Schoenoplectus | etuberculatus | | 2 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Mountain Rush | Juncus | balticus | | 1 | Juncaceae | Poales | Magnoliopsida | Plantae |
| Bayonet Rush | Juncus | militaris | | 1 | Juncaceae | Poales | Magnoliopsida | Plantae |
| Fringed Brome | Bromus | ciliatus | | 4 | Poaceae | Poales | Magnoliopsida | Plantae |
| Arctic Brome | Bromus | kalmii | | 18 | Poaceae | Poales | Magnoliopsida | Plantae |
| Sea Milkwort | Lysimachia | maritima | | 2 | Primulaceae | Primulales | Magnoliopsida | Plantae |
| Chaffweed | Lysimachia | minima | | 6 | Primulaceae | Primulales | Magnoliopsida | Plantae |
| Canada Anemone | Anemonastrum | canadense | | 3 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Curlyheads | Clematis | ochroleuca | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Pennsylvania Buttercup | Ranunculus | pensylvanicus | | 2 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Bebb Willow | Salix | bebbiana | | 4 | Salicaceae | Salicales | Magnoliopsida | Plantae |
| Shining Willow | Salix | lucida | | 2 | Salicaceae | Salicales | Magnoliopsida | Plantae |
| Drummond's St. Johnswort | Hypericum | drummondii | | 1 | Hypericaceae | Theales | Magnoliopsida | Plantae |
| Narrow-leaved Pinweed | Lechea | tenuifolia | | 5 | Cistaceae | Violales | Magnoliopsida | Plantae |
| Common Juniper | Juniperus | communis | | 2 | Cupressaceae | Pinales | Pinopsida | Plantae |
| Lanceleaf Grapefern | Botrychium | angustisegmentum | | 0 | Ophioglossaceae | Ophioglossales | Psilotopsida | Plantae |
| Little Grapefern | Botrychium | simplex | | 3 | Ophioglossaceae | Ophioglossales | Psilotopsida | Plantae |
| Leathery Grapefern | Sceptridium | multifidum | | 1 | Ophioglossaceae | Ophioglossales | Psilotopsida | Plantae |