| Redside Dace | Clinostomus | elongatus | | 2 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Blackbanded Sunfish | Enneacanthus | chaetodon | | 7 | Centrarchidae | Perciformes | Actinopterygii | Animalia |
| Hellbender | Cryptobranchus | alleganiensis | | 16 | Cryptobranchidae | Caudata | Amphibia | Animalia |
| Green Salamander | Aneides | aeneus | | 41 | Plethodontidae | Caudata | Amphibia | Animalia |
| Black Rail | Laterallus | jamaicensis | | 61 | Rallidae | Gruiformes | Aves | Animalia |
| Tidewater Mucket | Atlanticoncha | ochracea | | 17 | Unionidae | Unionoida | Bivalvia | Animalia |
| Yellow Lampmussel | Lampsilis | cariosa | | 12 | Unionidae | Unionoida | Bivalvia | Animalia |
| Eastern Pine Barrens Tiger Beetle | Cicindelidia | abdominalis | | 8 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Ghost Tiger Beetle | Ellipsoptera | lepida | | 12 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Eastern Beach Tiger Beetle (dorsalis) | Habroscelimorpha | dorsalis dorsalis | | 23 | Cicindelidae | Coleoptera | Insecta | Animalia |
| Eastern Beach Tiger Beetle (media) | Habroscelimorpha | dorsalis media | | 9 | Cicindelidae | Coleoptera | Insecta | Animalia |
| American Bumble Bee | Bombus | pensylvanicus | | 370 | Apidae | Hymenoptera | Insecta | Animalia |
| No Common Name | Zanclognatha | dentata | | 29 | Erebidae | Lepidoptera | Insecta | Animalia |
| Hessel's Hairstreak | Callophrys | hesseli | | 3 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| King's Hairstreak | Satyrium | kingi | | 25 | Lycaenidae | Lepidoptera | Insecta | Animalia |
| No Common Name | Hadena | ectypa | | 3 | Noctuidae | Lepidoptera | Insecta | Animalia |
| No Common Name | Lithophane | lemmeri | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| No Common Name | Meropleon | ambifusca | | 19 | Noctuidae | Lepidoptera | Insecta | Animalia |
| No Common Name | Meropleon | titan | | 49 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Hercules’ Club Borer Moth | Papaipema | araliae | | 4 | Noctuidae | Lepidoptera | Insecta | Animalia |
| No Common Name | Coelodasys | apicalis | | 5 | Notodontidae | Lepidoptera | Insecta | Animalia |
| Northern Metalmark | Calephelis | borealis | | 202 | Riodinidae | Lepidoptera | Insecta | Animalia |
| Slender Clearwing | Hemaris | gracilis | | 2 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Coppery Emerald | Somatochlora | georgiana | | 5 | Corduliidae | Odonata | Insecta | Animalia |
| Green-faced Clubtail | Hylogomphus | viridifrons | | 25 | Gomphidae | Odonata | Insecta | Animalia |
| Rapids Clubtail | Phanogomphus | quadricolor | | 56 | Gomphidae | Odonata | Insecta | Animalia |
| No Common Name | Protoptila | georgiana | | 2 | Glossosomatidae | Trichoptera | Insecta | Animalia |
| No Common Name | Hydropsyche | brunneipennis | | 1 | Hydropsychidae | Trichoptera | Insecta | Animalia |
| No Common Name | Hydropsyche | hoffmani | | 0 | Hydropsychidae | Trichoptera | Insecta | Animalia |
| Franz's Cave Amphipod | Stygobromus | franzi | | 21 | Crangonyctidae | Amphipoda | Malacostraca | Animalia |
| Shenandoah Valley Cave Amphipod | Stygobromus | gracilipes | | 7 | Crangonyctidae | Amphipoda | Malacostraca | Animalia |
| Pizzini's Amphipod | Stygobromus | pizzinii | | 6 | Crangonyctidae | Amphipoda | Malacostraca | Animalia |
| No Common Name | Caecidotea | vandeli | | 8 | Asellidae | Isopoda | Malacostraca | Animalia |
| Blue Whale | Balaenoptera | musculus | | 1 | Balaenopteridae | Cetacea | Mammalia | Animalia |
| Fin Whale | Balaenoptera | physalus | | 15 | Balaenopteridae | Cetacea | Mammalia | Animalia |
| Sperm Whale | Physeter | macrocephalus | | 13 | Physeteridae | Cetacea | Mammalia | Animalia |
| Silver-haired Bat | Lasionycteris | noctivagans | | 19 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Eastern Red Bat | Lasiurus | borealis | | 145 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Hoary Bat | Lasiurus | cinereus | | 11 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Allegheny Woodrat | Neotoma | magister | | 48 | Muridae | Rodentia | Mammalia | Animalia |
| Blue Wild indigo | Baptisia | australis | | 61 | Fabaceae | Fabales | Magnoliopsida | Plantae |