| Spotfin Shiner | Cyprinella | spiloptera | | 1 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Pearl Dace | Margariscus | margarita | | 1 | Cyprinidae | Cypriniformes | Actinopterygii | Animalia |
| Blue Ridge Sculpin | Cottus | caeruleomentum | | 1 | Cottidae | Scorpaeniformes | Actinopterygii | Animalia |
| Potomac Sculpin | Cottus | girardi | | 1 | Cottidae | Scorpaeniformes | Actinopterygii | Animalia |
| Checkered Sculpin | Cottus | undescribed | | 1 | Cottidae | Scorpaeniformes | Actinopterygii | Animalia |
| Margined Madtom | Noturus | insignis | | 1 | Ictaluridae | Siluriformes | Actinopterygii | Animalia |
| Cope's Gray Treefrog | Dryophytes | chrysoscelis | | 1 | Hylidae | Anura | Amphibia | Animalia |
| Long-tailed Salamander | Eurycea | longicauda | | 1 | Plethodontidae | Caudata | Amphibia | Animalia |
| Marbled Orbweaver | Araneus | marmoreus | | 1 | Araneidae | Araneae | Arachnida | Animalia |
| Shamrock Orbweaver | Araneus | trifolium | | 1 | Araneidae | Araneae | Arachnida | Animalia |
| Arrowshaped Micrathena | Micrathena | sagittata | | 1 | Araneidae | Araneae | Arachnida | Animalia |
| No Common Name | Leiobunum | vittatum | | 1 | Sclerosomatidae | Opiliones | Arachnida | Animalia |
| Bald Eagle | Haliaeetus | leucocephalus | | 1 | Accipitridae | Accipitriformes | Aves | Animalia |
| Eastern Towhee | Pipilo | erythrophthalmus | | 1 | Emberizidae | Passeriformes | Aves | Animalia |
| Field Sparrow | Spizella | pusilla | | 2 | Emberizidae | Passeriformes | Aves | Animalia |
| Snowy Owl | Bubo | scandiacus | | 2 | Strigidae | Strigiformes | Aves | Animalia |
| Dusky Arion | Arion | subfuscus | | 1 | Arionidae | Stylommatophora | Gastropoda | Animalia |
| Leopard Slug | Limax | maximus | | 1 | Limacidae | Stylommatophora | Gastropoda | Animalia |
| Colorado Potato Beetle | Leptinotarsa | decemlineata | | 1 | Chrysomelidae | Coleoptera | Insecta | Animalia |
| Silky Laphria | Laphria | sericea | | 1 | Asilidae | Diptera | Insecta | Animalia |
| Tiger Bee Fly | Xenox | tigrinus | | 1 | Bombyliidae | Diptera | Insecta | Animalia |
| Giant Eastern Crane Fly | Pedicia | albivitta | | 1 | Pediciidae | Diptera | Insecta | Animalia |
| Peacock Fly | Callopistromyia | annulipes | | 2 | Ulidiidae | Diptera | Insecta | Animalia |
| Rhododendron Leafhopper | Graphocephala | fennahi | | 1 | Cicadellidae | Hemiptera | Insecta | Animalia |
| Green Stink Bug | Chinavia | hilaris | | 1 | Pentatomidae | Hemiptera | Insecta | Animalia |
| Wheel Bug | Arilus | cristatus | | 1 | Reduviidae | Hemiptera | Insecta | Animalia |
| Pale Green Assassin Bug | Zelus | luridus | | 1 | Reduviidae | Hemiptera | Insecta | Animalia |
| Wool Sower | Callirhytis | seminator | | 1 | Cynipidae | Hymenoptera | Insecta | Animalia |
| German Yellowjacket | Vespula | germanica | | 1 | Vespidae | Hymenoptera | Insecta | Animalia |
| Angel Moth | Olceclostera | angelica | | 1 | Apatelodidae | Lepidoptera | Insecta | Animalia |
| Vagabond Crambus Moth | Agriphila | vulgivagella | | 1 | Crambidae | Lepidoptera | Insecta | Animalia |
| Biden's Grass-veneer Moth | Crambus | bidens | | 1 | Crambidae | Lepidoptera | Insecta | Animalia |
| Pasture Grass-veneer Moth | Crambus | saltuellus | | 1 | Crambidae | Lepidoptera | Insecta | Animalia |
| Arched Hooktip Moth | Drepana | arcuata | | 1 | Drepanidae | Lepidoptera | Insecta | Animalia |
| Milkweed Tussock Moth | Euchaetes | egle | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Clymene Moth | Haploa | clymene | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Leconte's Haploa Moth | Haploa | lecontei | | 2 | Erebidae | Lepidoptera | Insecta | Animalia |
| Green Cloverworm Moth | Hypena | scabra | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Giant Leopard Moth | Hypercompe | scribonia | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Hickory Tussock Moth | Lophocampa | caryae | | 2 | Erebidae | Lepidoptera | Insecta | Animalia |
| Spongy Moth | Lymantria | dispar | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Faint-spotted Palthis Moth | Palthis | asopialis | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Maple Looper Moth | Parallelia | bistriaris | | 1 | Erebidae | Lepidoptera | Insecta | Animalia |
| Straw Besma Moth | Besma | endropiaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Oak Besma Moth | Besma | quercivoraria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Peppered Moth | Biston | betularia | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Pale Beauty Moth | Campaea | perlata | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Chain-dotted Geometer Moth | Cingilia | catenaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Badwing Moth | Dyspteris | abortivaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Curve-toothed Geometer Moth | Eutrapela | clemataria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Bent-line Gray Moth | Iridopsis | larvaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Curve-lined Looper Moth | Lambdina | fervidaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Lesser Maple Spanworm Moth | Macaria | pustularia | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| White-fringed Emerald Moth | Nemoria | mimosaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| False Hemlock Looper Moth | Nepytia | canosaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Half-wing Moth | Phigalia | titea | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Friendly Probole Moth | Probole | amicaria | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Large Lace-bordered Moth | Scopula | limboundata | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| White Slant-line Moth | Tetracis | cachexiata | | 1 | Geometridae | Lepidoptera | Insecta | Animalia |
| Forest Tent Caterpillar Moth | Malacosoma | disstria | | 1 | Lasiocampidae | Lepidoptera | Insecta | Animalia |
| Shagreened Slug Moth | Apoda | biguttata | | 1 | Limacodidae | Lepidoptera | Insecta | Animalia |
| Spiny Oak-slug Moth | Euclea | delphinii | | 1 | Limacodidae | Lepidoptera | Insecta | Animalia |
| Soybean Looper Moth | Chrysodeixis | includens | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Implicit Arches Moth | Lacinipolia | implicata | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Eastern Panthea Moth | Panthea | furcilla | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Hebrew Moth | Polygrammate | hebraeicum | | 1 | Noctuidae | Lepidoptera | Insecta | Animalia |
| Yellow-necked Caterpillar Moth | Datana | ministra | | 1 | Notodontidae | Lepidoptera | Insecta | Animalia |
| White-dotted Prominent Moth | Nadata | gibbosa | | 1 | Notodontidae | Lepidoptera | Insecta | Animalia |
| Morning-glory Prominent Moth | Schizura | ipomaeae | | 1 | Notodontidae | Lepidoptera | Insecta | Animalia |
| Morning-glory Plume Moth | Emmelina | monodactyla | | 1 | Pterophoridae | Lepidoptera | Insecta | Animalia |
| Stored Grain Moth | Aglossa | caprealis | | 1 | Pyralidae | Lepidoptera | Insecta | Animalia |
| Clover Hayworm Moth | Hypsopygia | costalis | | 1 | Pyralidae | Lepidoptera | Insecta | Animalia |
| Luna Moth | Actias | luna | | 1 | Saturniidae | Lepidoptera | Insecta | Animalia |
| Pink-striped Oakworm Moth | Anisota | virginiensis | | 1 | Saturniidae | Lepidoptera | Insecta | Animalia |
| Waved Sphinx | Ceratomia | undulosa | | 2 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Virginia Creeper Sphinx | Darapsa | myron | | 1 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Blinded Sphinx | Paonias | excaecata | | 1 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Laurel Sphinx | Sphinx | kalmiae | | 1 | Sphingidae | Lepidoptera | Insecta | Animalia |
| Ailanthus Webworm Moth | Atteva | aurea | | 1 | Yponomeutidae | Lepidoptera | Insecta | Animalia |
| Grapeleaf Skeletonizer Moth | Harrisina | americana | | 1 | Zygaenidae | Lepidoptera | Insecta | Animalia |
| No Common Name | Panorpa | helena | | 1 | Panorpidae | Mecoptera | Insecta | Animalia |
| Eastern Dobsonfly | Corydalus | cornutus | | 1 | Corydalidae | Megaloptera | Insecta | Animalia |
| Shadow Darner | Aeshna | umbrosa | | 1 | Aeshnidae | Odonata | Insecta | Animalia |
| Fawn Darner | Boyeria | vinosa | | 2 | Aeshnidae | Odonata | Insecta | Animalia |
| Dragonhunter | Hagenius | brevistylus | | 1 | Gomphidae | Odonata | Insecta | Animalia |
| Upland Burrowing Crayfish | Cambarus | dubius | | 2 | Cambaridae | Decapoda | Malacostraca | Animalia |
| Spiny-cheeked Crayfish | Faxonius | limosus | | 1 | Cambaridae | Decapoda | Malacostraca | Animalia |
| Black Bear | Ursus | americanus | | 1 | Ursidae | Carnivora | Mammalia | Animalia |
| Silver-haired Bat | Lasionycteris | noctivagans | | 1 | Vespertilionidae | Chiroptera | Mammalia | Animalia |
| Red Squirrel | Tamiasciurus | hudsonicus | | 1 | Sciuridae | Rodentia | Mammalia | Animalia |
| Woodland Jumping Mouse | Napaeozapus | insignis | | 1 | Zapodidae | Rodentia | Mammalia | Animalia |
| Queen Snake | Regina | septemvittata | | 1 | Colubridae | Squamata | Reptilia | Animalia |
| American Caesar's Amanita | Amanita | jacksonii | | 1 | Amanitaceae | Agaricales | Agaricomycetes | Fungi |
| Golden Fairy Spindle | Clavulinopsis | fusiformis | | 1 | Clavariaceae | Agaricales | Agaricomycetes | Fungi |
| Shaggy Mane | Coprinus | comatus | | 1 | Coprinaceae | Agaricales | Agaricomycetes | Fungi |
| Jack O' Lantern Mushroom | Omphalotus | illudens | | 1 | Omphalotaceae | Agaricales | Agaricomycetes | Fungi |
| Hen of the Woods | Grifola | frondosa | | 1 | Grifolaceae | Polyporales | Agaricomycetes | Fungi |
| Sulfur Shelf | Laetiporus | sulphureus | | 1 | Laetiporaceae | Polyporales | Agaricomycetes | Fungi |
| Lacquered Polypore | Ganoderma | lucidum | | 1 | Polyporaceae | Polyporales | Agaricomycetes | Fungi |
| Crown-tipped Coral | Artomyces | pyxidatus | | 1 | Auriscalpiaceae | Russulales | Agaricomycetes | Fungi |
| British Soldiers | Cladonia | cristatella | | 1 | Cladoniaceae | Lecanorales | Lecanoromycetes | Fungi |
| Pink Earth Lichen | Dibaeis | baeomyces | | 1 | Icmadophilaceae | Pertusariales | Lecanoromycetes | Fungi |
| Greencap Jellybaby | Leotia | lubrica | | 1 | Leotiaceae | Leotiales | Leotiomycetes | Fungi |
| Schreber's Big Red Stem Moss | Pleurozium | schreberi | | 1 | Hylocomiaceae | Hypnales | Bryopsida | Plantae |
| Blue Ground Cedar | Diphasiastrum | tristachyum | | 1 | Lycopodiaceae | Lycopodiales | Lycopodiopsida | Plantae |
| Dwarf Ginseng | Panax | trifolius | | 1 | Araliaceae | Apiales | Magnoliopsida | Plantae |
| Common Jack in the Pulpit | Arisaema | triphyllum | | 1 | Araceae | Arales | Magnoliopsida | Plantae |
| Ramps | Allium | tricoccum | | 1 | Amaryllidaceae | Asparagales | Magnoliopsida | Plantae |
| Starch Grape Hyacinth | Muscari | neglectum | | 1 | Asparagaceae | Asparagales | Magnoliopsida | Plantae |
| Hairy Solomon's-seal | Polygonatum | pubescens | | 1 | Asparagaceae | Asparagales | Magnoliopsida | Plantae |
| Common Sneezeweed | Helenium | autumnale | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Rattlesnake Weed | Hieracium | venosum | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Whorled Wood Aster | Oclemena | acuminata | | 1 | Asteraceae | Asterales | Magnoliopsida | Plantae |
| Cardinal Flower | Lobelia | cardinalis | | 1 | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| Great Blue Lobelia | Lobelia | siphilitica | | 1 | Campanulaceae | Asterales | Magnoliopsida | Plantae |
| Broad-leaved Toothwort | Cardamine | diphylla | | 1 | Brassicaceae | Brassicales | Magnoliopsida | Plantae |
| Widowsfrill | Silene | stellata | | 1 | Caryophyllaceae | Caryophyllales | Magnoliopsida | Plantae |
| Round-leaved Sundew | Drosera | rotundifolia | | 1 | Droseraceae | Caryophyllales | Magnoliopsida | Plantae |
| Asiatic Tearthumb | Persicaria | perfoliata | | 1 | Polygonaceae | Caryophyllales | Magnoliopsida | Plantae |
| Japanese Knotweed | Reynoutria | japonica | | 1 | Polygonaceae | Caryophyllales | Magnoliopsida | Plantae |
| Trailing Arbutus | Epigaea | repens | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Minniebush | Menziesia | pilosa | | 1 | Ericaceae | Ericales | Magnoliopsida | Plantae |
| Northern Pitcherplant | Sarracenia | purpurea | | 1 | Sarraceniaceae | Ericales | Magnoliopsida | Plantae |
| Beaked Hazelnut | Corylus | cornuta | | 1 | Betulaceae | Fagales | Magnoliopsida | Plantae |
| American Chestnut | Castanea | dentata | | 2 | Fagaceae | Fagales | Magnoliopsida | Plantae |
| Orange Jewelweed | Impatiens | capensis | | 2 | Balsaminaceae | Geraniales | Magnoliopsida | Plantae |
| Yellow Jewelweed | Impatiens | pallida | | 2 | Balsaminaceae | Geraniales | Magnoliopsida | Plantae |
| Wild Geranium | Geranium | maculatum | | 1 | Geraniaceae | Geraniales | Magnoliopsida | Plantae |
| Violet Woodsorrel | Oxalis | violacea | | 1 | Oxalidaceae | Geraniales | Magnoliopsida | Plantae |
| Two-leaved Watermilfoil | Myriophyllum | heterophyllum | | 1 | Haloragaceae | Haloragales | Magnoliopsida | Plantae |
| Short-spiked Watermilfoil | Myriophyllum | sibiricum | | 1 | Haloragaceae | Haloragales | Magnoliopsida | Plantae |
| American Witch-hazel | Hamamelis | virginiana | | 1 | Hamamelidaceae | Hamamelidales | Magnoliopsida | Plantae |
| Southern Waternymph | Najas | guadalupensis | | 1 | Hydrocharitaceae | Hydrocharitales | Magnoliopsida | Plantae |
| Common Bugle | Ajuga | reptans | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Purple Deadnettle | Lamium | purpureum | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| White Bergamot | Monarda | clinopodia | | 1 | Lamiaceae | Lamiales | Magnoliopsida | Plantae |
| Bear Corn | Conopholis | americana | | 1 | Orobanchaceae | Lamiales | Magnoliopsida | Plantae |
| White Turtlehead | Chelone | glabra | | 2 | Plantaginaceae | Lamiales | Magnoliopsida | Plantae |
| Sessile-leaved Bellwort | Uvularia | sessilifolia | | 1 | Colchicaceae | Liliales | Magnoliopsida | Plantae |
| Yellow Trout Lily | Erythronium | americanum | | 1 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Turk's-cap Lily | Lilium | superbum | | 1 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Indian Cucumber | Medeola | virginiana | | 1 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| Yellow Fairybells | Prosartes | lanuginosa | | 1 | Liliaceae | Liliales | Magnoliopsida | Plantae |
| White Trillium | Trillium | grandiflorum | | 1 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Painted Trillium | Trillium | undulatum | | 1 | Melanthiaceae | Liliales | Magnoliopsida | Plantae |
| Smooth Carrionflower | Smilax | herbacea | | 1 | Smilacaceae | Liliales | Magnoliopsida | Plantae |
| Waterthread Pondweed | Potamogeton | diversifolius | | 1 | Potamogetonaceae | Najadales | Magnoliopsida | Plantae |
| Small Pondweed (pusillus) | Potamogeton | pusillus | | 1 | Potamogetonaceae | Najadales | Magnoliopsida | Plantae |
| Robbins's Pondweed | Potamogeton | robbinsii | | 1 | Potamogetonaceae | Najadales | Magnoliopsida | Plantae |
| Spiral Pondweed | Potamogeton | spirillus | | 1 | Potamogetonaceae | Najadales | Magnoliopsida | Plantae |
| Downy Rattlesnake Plantain | Goodyera | pubescens | | 1 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Green-fringed Orchid | Platanthera | lacera | | 1 | Orchidaceae | Orchidales | Magnoliopsida | Plantae |
| Bloodroot | Sanguinaria | canadensis | | 1 | Papaveraceae | Papaverales | Magnoliopsida | Plantae |
| Tawny Cottongrass | Eriophorum | virginicum | | 1 | Cyperaceae | Poales | Magnoliopsida | Plantae |
| Fringed Polygala | Polygaloides | paucifolia | | 1 | Polygalaceae | Polygalales | Magnoliopsida | Plantae |
| Starflower | Lysimachia | borealis | | 1 | Primulaceae | Primulales | Magnoliopsida | Plantae |
| White Baneberry | Actaea | pachypoda | | 2 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Mountain Bugbane | Actaea | podocarpa | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Wood Anemone | Anemone | quinquefolia | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Yellow Marsh Marigold | Caltha | palustris | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Threeleaf Goldthread | Coptis | trifolia | | 1 | Ranunculaceae | Ranunculales | Magnoliopsida | Plantae |
| Indian Strawberry | Potentilla | indica | | 1 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Black Cherry | Prunus | serotina | | 1 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Wineberry | Rubus | phoenicolasius | | 2 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| American Mountain Ash | Sorbus | americana | | 1 | Rosaceae | Rosales | Magnoliopsida | Plantae |
| Creeping Bluet | Houstonia | serpyllifolia | | 1 | Rubiaceae | Rubiales | Magnoliopsida | Plantae |
| Silky Willow | Salix | sericea | | 1 | Salicaceae | Salicales | Magnoliopsida | Plantae |
| Common Bladderwort | Utricularia | macrorhiza | | 1 | Lentibulariaceae | Scrophulariales | Magnoliopsida | Plantae |
| Halberd-leaved Yellow Violet | Viola | hastata | | 1 | Violaceae | Violales | Magnoliopsida | Plantae |
| Eastern Hemlock | Tsuga | canadensis | | 1 | Pinaceae | Pinales | Pinopsida | Plantae |
| Common Polypody | Polypodium | virginianum | | 1 | Polypodiaceae | Polypodiales | Polypodiopsida | Plantae |